Hello, My name is Brandon. Thanks for joining the Zoom Community! Once you enable breakout rooms you can click on the breakout rooms button in your meeting. Preferably after all of your participants have joined and then you can assign the participants to a room or allow your participants to choose which room that they would like to go in.
You can also pre-assign your participants to breakout rooms, but again, I stress that you should wait until a majority of your participants are in the main session prior to launching.